Blank Solution
| Column | Topsil C18, 4.6×250mm, 5μm |
| Detection | 254nm |
| Temperature | 43℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 1ul |
Mobile Phase
A: 0.1mol/L sodium acetate(pH6.5) : Acetonitrile = 93:7
B: Acetonitrile:Water=20:80
| Time | A% | B% |
| 0 | 50 | 50 |
| 20 | 20 | 80 |
| 22 | 0 | 100 |
| 35 | 0 | 100 |
| 36 | 50 | 50 |
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