| Column | Ultisil® Polar-RP（4.6×100mm，3μm) Ultisil® Polar-RP（4.6×150mm，3μm) Ultisil® Polar-RP（4.6×250mm，5μm) |
| Mobile Phase | See the table below for details (Mobile phase A: Take 3.4g potassium dihydrogen phosphate and 8.94g disodium hydrogen phosphate, add 2000mL Wahaha water to dissolve, adjust the pH to 6.8 with phosphoric acid, mix 700mL of the above solution with 250mL chromatography-grade methanol and 50mL chromatography-grade acetonitrile, shake well, and ultrasonic degassed to obtain. Mobile phase B: 300mL of the above configured phosphate solution was mixed with 700mL of chromatographic grade acetonitrile and shaken well to obtain.) |
| Detection | 230nm |
| Temperature | 25℃ |
| Flow Rate | Ultisil® Polar-RP（4.6×100mm，3μm) 1.0ml/min Ultisil® Polar-RP（4.6×150mm，3μm) 1.0ml/min Ultisil® Polar-RP（4.6×250mm，5μm) 1.8ml/min |
| Injection Volume | 20 μL |
Mobile Phase
| Time (min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 100 | 0 |
| 10 | 100 | 0 |
| 30 | 50 | 50 |
| 40 | 0 | 100 |
| 45 | 0 | 100 |
| 45.1 | 100 | 0 |
| 55 | 100 | 0 |
If you have any problem or require further information, please contact info@welchmat.com.
0 Comments