ColumnUltisil® Polar-RP(4.6×100mm,3μm)
Ultisil® Polar-RP(4.6×150mm,3μm)
Ultisil® Polar-RP(4.6×250mm,5μm)
Mobile PhaseSee the table below for details
(Mobile phase A: Take 3.4g potassium dihydrogen phosphate and 8.94g disodium
hydrogen phosphate, add 2000mL Wahaha water to dissolve, adjust the pH to 6.8
with phosphoric acid, mix 700mL of the above solution with 250mL
chromatography-grade methanol and 50mL chromatography-grade acetonitrile,
shake well, and ultrasonic degassed to obtain.
Mobile phase B: 300mL of the above configured phosphate solution was mixed
with 700mL of chromatographic grade acetonitrile and shaken well to obtain.)
Detection230nm
Temperature25℃
Flow RateUltisil® Polar-RP(4.6×100mm,3μm) 1.0ml/min
Ultisil® Polar-RP(4.6×150mm,3μm) 1.0ml/min
Ultisil® Polar-RP(4.6×250mm,5μm) 1.8ml/min
Injection Volume20 μL

Mobile Phase

Time (min)Mobile phase A(%)Mobile phase B(%)
01000
101000
305050
400100
450100
45.11000
551000

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