Reference substance
Vitamin K1 Standard Sample (Concentration 2 ug/mL)
| Column | Ultisil® AQ-C18 4.6×250 mm, 5 μm Ultisil® Zn 4.6×50mm |
| Mobile Phase | 900mL Methanol (4.6), 100 mL dichloromethane (4.7), 0.3mL glacial acetic acid (4.8), 1.5g zinc chloride (4.9), 0.5g Sodium acetate anhydrous (4.10), filter with 0.45μm membrane after dissolution |
| Detection | 243 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 µl |
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