Reference substance

 Vitamin K1 Standard Sample (Concentration 2 ug/mL)

ColumnUltisil® AQ-C18 4.6×250 mm, 5 μm  Ultisil® Zn 4.6×50mm
Mobile Phase900mL Methanol (4.6), 100 mL dichloromethane (4.7), 0.3mL glacial acetic acid (4.8), 1.5g zinc chloride (4.9), 0.5g Sodium acetate anhydrous (4.10), filter with 0.45μm membrane after dissolution
Detection243 nm
Temperature30 ℃
Flow Rate1.0 ml/min
Injection Volume20 µl

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