Determination of vitamin B6 related substances and content by Ultisil XB-C18

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ColumnUltisil® XB-C18（4.6×250mm，5μm)
Mobile Phase0.04% sodium pentanesulfonate solution (adjust pH to 3.0 with glacial acetic
acid)/methanol=85/15
Detection291nm
Temperature35℃
Flow Rate1.0 ml/min
Injection Volume10 μL

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