Vitamin B6, also known as pyridoxine, includes pyridoxine, pyridoxal and pyridoxamine. It exists in the form of phosphate in the body. It is a water-soluble vitamin. It is a component of some coenzymes in the human body and participates in a variety of metabolic reactions, especially closely related to amino acid metabolism. Clinical application of vitamin B6 preparation in the prevention and treatment of pregnancy vomiting and radiation sickness vomiting. Vitamin B6 is abundant in yeast, liver, grains, meat, fish, eggs, beans and peanuts.
Chromatographic conditions
| Column | Ultisil® AQ-C18 (4.6×150mm,5μm) |
| Detection | Excitation wavelength: 293nm Emission wavelength: 395nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10μL |
| Note | / |
| Mobile Phase | ||
| Time(min) | Mobile phase A (%) | Methanol (%) |
| 0 | 100 | 0 |
| 20 | 100 | 0 |
| 30 | 65 | 35 |
| 45 | 62 | 38 |
| 46 | 50 | 50 |
| 50 | 50 | 50 |
| 51 | 100 | 0 |
| 75 | 100 | 0 |
Chromatographic Data
| Material | RT | Area | Height | Plate no | Tailing Factor | Resolution |
| Pyridoxal | 15.295 | 55641163 | 2708855 | 12581 | 0.999 | – |
| Pyridoxal | 22.436 | 87149062 | 2970285 | 13341 | 0.996 | 10.801 |
| Pyridoxal | 36.602 | 73230997 | 9202969 | 445781 | 1.087 | 28.438 |
| Total | 216021223 | 14882108 |
Result
With Welch Ultisil®AQ-C18（4.6 × 150mm，5 μ m),under this chromatographic condition, it can meet the requirements of detection.
If you have any problem or require further information, please contact info@welchmat
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