Mixed standard solution-enlarged view
| Column | Ultisil® AQ C18（ 4.6×150mm，5μm) |
| Detection | Excitation wavelength: 293nm , Emission wavelength: 395nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
| Time(min） | Mobile phase A (%) | Mobile phase B (%) |
| 0 | 100 | 0 |
| 20 | 100 | 0 |
| 30 | 65 | 35 |
| 45 | 62 | 38 |
| 46 | 50 | 50 |
| 50 | 50 | 50 |
| 51 | 100 | 0 |
| 75 | 100 | 0 |
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