4mg/L standard soluiton
| No. | Peak Name | Rt | Area | Height | Area | Tailing | Plates |
| min | mAU*min | mAU | % | EP | (USP) | ||
| 1 | Vitamin B12 | 6.266 | 0.8547 | 5.32 | 100 | 0.92 | 10002 |
| Column | Xtimate® C18, 4.6×250mm, 5μm |
| Mobile Phase | Mobile phase A: weigh 0.87g dipotassium hydrogen phosphate and 0.41g dipotassium phosphate accurately, then dissolve with water. Diluted to 1000mL with 125mL acetonitrile. Mobile phase B: Water-acetonitrile-phosphoric acid =499:499:2 |
| Wavelength | 361 nm |
| Temperature | 30℃ |
| Flow Rate | 1.2 ml/min |
| Injection Volume | 20 μl |
| Notes | Sensitive to injection volume and solvent for dissolving sample |
Mobile phase
| Time | Mobile Phase A | Mobile Phase B |
| 0 | 100 | 0 |
| 13 | 100 | 0 |
| 18 | 0 | 100 |
| 23 | 100 | 0 |
| 25 | 100 | 0 |
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