Determination of Vitamin B12 in Functional Beverage

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4mg/L standard soluiton

No.Peak NameRtAreaHeightAreaTailingPlates
  minmAU*minmAU%EP(USP)
1Vitamin B126.2660.85475.321000.9210002
ColumnXtimate® C18, 4.6×250mm, 5μm
Mobile PhaseMobile phase A: weigh 0.87g dipotassium hydrogen phosphate and 0.41g dipotassium phosphate accurately, then dissolve with water. Diluted to 1000mL with 125mL acetonitrile.
Mobile phase B: Water-acetonitrile-phosphoric acid =499:499:2
Wavelength361 nm
Temperature30℃
Flow Rate1.2 ml/min
Injection Volume20 μl
NotesSensitive to injection volume and solvent for dissolving sample

Mobile phase

TimeMobile Phase AMobile Phase B
01000
131000
180100
231000
251000

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