4mg/L standard soluiton
No. | Peak Name | Rt | Area | Height | Area | Tailing | Plates |
min | mAU*min | mAU | % | EP | (USP) | ||
1 | Vitamin B12 | 6.266 | 0.8547 | 5.32 | 100 | 0.92 | 10002 |
Column | Xtimate® C18, 4.6×250mm, 5μm |
Mobile Phase | Mobile phase A: weigh 0.87g dipotassium hydrogen phosphate and 0.41g dipotassium phosphate accurately, then dissolve with water. Diluted to 1000mL with 125mL acetonitrile. Mobile phase B: Water-acetonitrile-phosphoric acid =499:499:2 |
Wavelength | 361 nm |
Temperature | 30℃ |
Flow Rate | 1.2 ml/min |
Injection Volume | 20 μl |
Notes | Sensitive to injection volume and solvent for dissolving sample |
Mobile phase
Time | Mobile Phase A | Mobile Phase B |
0 | 100 | 0 |
13 | 100 | 0 |
18 | 0 | 100 |
23 | 100 | 0 |
25 | 100 | 0 |
If you have any problem or require further information, please contact info@welchmat.com.
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