Blank solvent
| Column | Xtimate® C18（4.0×250mm，5μm) |
| Detection | 248nm |
| Temperature | 45℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 25ul |
| Note | / |
Mobile Phase
| Time(min） | Mobile phase A (%) | Mobile phase B (%) |
| 0-2 | 90 | 10 |
| 2-27 | 90-70 | 10-30 |
| 27-35 | 70-50 | 30-50 |
| 35-42 | 50 | 50 |
If you have any problem or require further information, please contact info@welchmat.com.
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