Assay solution

ColumnUltisil® AQ-C18 4.6×250 mm, 5 μm
Detection240 nm
Temperature40 ℃
Flow Rate1.0 ml/min
Injection Volume20 µl

Mobile Phase

Phosphate buffer/acetonitrile=60/40

TimePhosphate BufferAcetonitrile
07525
37525
256040
403565
503565
50.17525
657525

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