Determination of VB12 in Infant Milk Powder

Published by News Editor on

1mg/L VB12

No.Peak NameRtAreaHeightAreaAsymmetryResolutionPlates
  minmAU*minmAU%(EP)(EP)(EP)
1VB128.0750.2361.251100.00n.a.0.899908
ColumnXtimate® C18, 4.6×250mm,5μm
Mobile phaseMobile phase A: weigh 0.87g dipotassium hydrogen phosphate and 0.41g dipotassium hydrogen phosphate accurately, then dissolve them with water. Add 125mL of acetonitrile and dilute with water to1000 mL.
Mobile phase B: water – acetonitrile – phosphoric acid =499-499-2
Wavelength361 nm
Temperature30℃
Flow Rate1.2 mL/min
Injection Volume20 μL

Mobile Phase

TimeMobile phase AMobile phase B
01000
131000
180100
231000
251000

If you have any problem or require further information, please contact info@welchmat.com.


0 Comments

Leave a Reply

Avatar placeholder

Your email address will not be published. Required fields are marked *