Stansdard reference of VB12
| Column | Xtimate® C18, 4.6×250mm,5μm |
| Mobile Phase | A: weigh 0.87g dipotassium hydrogen phosphate and 0.41g dipotassium hydrogen phosphate accurately, then dissolve them with water. Add 125mL of acetonitrile and dilute with water to1000 mL. B:water – acetonitrile – phosphoric acid =499-499-2 |
| Wavelength | 361 nm |
| Temperature | 30℃ |
| Flow Rate | 1.2 mL/min |
| Injection Volume | 20 μl |
Mobile Phase
| Time | Mobile phase A | Mobile phase B |
| 0 | 100 | 0 |
| 13 | 100 | 0 |
| 18 | 0 | 100 |
| 23 | 100 | 0 |
| 25 | 100 | 0 |
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