Stansdard reference of VB12
Column | Xtimate® C18, 4.6×250mm,5μm |
Mobile Phase | A: weigh 0.87g dipotassium hydrogen phosphate and 0.41g dipotassium hydrogen phosphate accurately, then dissolve them with water. Add 125mL of acetonitrile and dilute with water to1000 mL. B:water – acetonitrile – phosphoric acid =499-499-2 |
Wavelength | 361 nm |
Temperature | 30℃ |
Flow Rate | 1.2 mL/min |
Injection Volume | 20 μl |
Mobile Phase
Time | Mobile phase A | Mobile phase B |
0 | 100 | 0 |
13 | 100 | 0 |
18 | 0 | 100 |
23 | 100 | 0 |
25 | 100 | 0 |
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