Stansdard reference of VB12
|Column||Xtimate® C18, 4.6×250mm,5μm|
|Mobile Phase||A: weigh 0.87g dipotassium hydrogen phosphate and 0.41g dipotassium hydrogen phosphate accurately, then dissolve them with water. Add 125mL of acetonitrile and dilute with water to1000 mL.|
B:water – acetonitrile – phosphoric acid =499-499-2
|Flow Rate||1.2 mL/min|
|Injection Volume||20 μl|
|Time||Mobile phase A||Mobile phase B|
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