Determination of VB12 in Health-care Food

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Stansdard reference of VB12

ColumnXtimate® C18, 4.6×250mm,5μm
Mobile PhaseA: weigh 0.87g dipotassium hydrogen phosphate and 0.41g dipotassium hydrogen phosphate accurately, then dissolve them with water. Add 125mL of acetonitrile and dilute with water to1000 mL.
B:water – acetonitrile – phosphoric acid =499-499-2
Wavelength361 nm
Temperature30℃
Flow Rate1.2 mL/min
Injection Volume20 μl

Mobile Phase

TimeMobile phase AMobile phase B
01000
131000
180100
231000
251000

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