| Column | Xtimate® C18(4.6*250mm,5μm) |
| Mobile Phase | See the table below for details |
| Detection | 200 nm/280 nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 50 μL |
| Note | 1. If 200 nm wavelength is selected, use higher quality chromatography grade methanol. 2. After comparative testing, the response of VB2 at 280nm is greater than 200nm, and the other five substances have higher response at 200nm wavelength. |
Mobile Phase: (A: 30 mmol/L potassium phosphorus dihydrogen (adjust pH to 5.0) B: methanol)
| Time (min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 95 | 5 |
| 15 | 77 | 23 |
| 20 | 50 | 50 |
| 25 | 50 | 50 |
| 25.01 | 95 | 5 |
| 35 | 95 | 5 |
If you have any problem or require further information, please contact info@welchmat.com.
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