ColumnUltisil® LP- C18(4.6×250mm,5μm)
Mobile PhaseSee the table below for details
Detection220nm
Temperature20℃
Flow Rate1.0 ml/min
Injection Volume10 μL

Mobile Phase:

Mobile phase A: Weigh 3.48g of dipotassium hydrogen phosphate, dissolve it in 1000ml of Wahaha water, adjust the pH to 7.0 with phosphoric acid, mix well, filter with suction, and degas by ultrasonic Mobile phase B: Acetonitrile.

Time (min)Mobile phase A(%)Mobile phase B(%)
09010
68416
107822
407525
505545
519010
659010

If you have any problem or require further information, please contact info@welchmat.com.


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