Sample solution enlarged
| Column | Ultisil® LP- C18（4.6×250mm，5μm) |
| Detection | 220nm |
| Temperature | 20℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
Mobile phase A: weigh 3.48g of dipotassium hydrogen phosphate, dissolve it in 1000ml water, adjust the pH to 7.0 with phosphoric acid, mix well, filter by suction, and degass by ultrasound;
Mobile phase B: acetonitrile
| Time(min) | Mobile phase A (%) | Acetonitrile (%) |
| 0 | 90 | 10 |
| 6 | 84 | 16 |
| 10 | 78 | 22 |
| 40 | 75 | 25 |
| 50 | 55 | 45 |
| 51 | 90 | 10 |
| 65 | 90 | 10 |
If you have any problem or require further information, please contact info@welchmat.com
0 Comments