Fenugreek is the seed of genus fenugreek in the legume family. It has the functions of warming kidney, invigorating , removing cold and dehumidifying. It is used to treat cold hernia, abdominal hypochasm fullness, cold and wet beriberi, kidney deficiency and lumbar acid, etc. In China, fenugreek belongs to the same food and medicine. Recent studies have shown that its seeds can reduce blood sugar and prevent hypertension.

In this article, Welch Ultisil®Plus C18 was used for the determination of fenugreek in accordance with the method of The Chinese Pharmacopoeia 2020 edition. The results can meet the detection requirements.

Chromatographic conditions

Column: Welch Ultisil® Plus C18 (4.6×250mm, 5μm).

Mobile phase: Methanol /0.05% sodium dodecyl sulfonate/glacial acetic acid =20/80/0.1;

Wavelength: 265nm;

Temperature: 25℃;

Flow rate: 1.0mL/min;

Injection volume: 10μL.

Spectrograms and data

Reference solution

Test solution


Using Welch Ultisil® Plus C18 (4.6×250mm, 5μm), the chromatographic conditions can meet the detection requirements.

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