| Column | Ultisil®LP-C18（4.6×250mm，5μm） |
| Mobile Phase | See the table below for details |
| Detection | 225nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
Mobile Phase:
Mobile phase A: (0.1% phosphoric acid solution) Mobile phase B: (acetonitrile)
| Time (min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 45 | 55 |
| 10 | 45 | 55 |
| 20 | 15 | 85 |
| 25 | 45 | 55 |
If you have any problem or require further information, please contact info@welchmat.com.
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