| Column | Ultisil® XB-C18 (4.6×250mm, 5μm) |
| Mobile Phase | Mobile phase: take 18.3g sodium dihydrogen phosphate and 2.3g sodium dodecyl sulfate and add 800mL silicon Haha water, mix well, ultrasonically degas, suction filtration, add 8.3mL triethylamine and mix well, adjust pH to 2.5 with phosphoric acid, add Wahaha water to 1000mL, shake well. Mix 600 mL of the above solution with 400 mL of chromatographic grade methanol, and ultrasonically degas to obtain. 0.23% sodium dodecyl sulfate solution/methanol=60/40 |
| Detection | 220nm |
| Temperature | 25℃ |
| Flow Rate | 0.9 ml/min |
| Injection Volume | 20 μL |
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