ColumnUltisil® XB-C18 (4.6×250mm, 5μm)
Mobile PhaseMobile phase: take 18.3g sodium dihydrogen phosphate and 2.3g sodium dodecyl
sulfate and add 800mL silicon
Haha water, mix well, ultrasonically degas, suction filtration, add 8.3mL
triethylamine and mix well, adjust pH to 2.5 with phosphoric acid, add Wahaha
water to 1000mL, shake well. Mix 600 mL of the above solution with 400 mL of
chromatographic grade methanol, and ultrasonically degas to obtain.
0.23% sodium dodecyl sulfate solution/methanol=60/40
Flow Rate0.9 ml/min
Injection Volume20 μL

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