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| Column | Ultisil®XB-C18（ 4.6×250mm，5μm) |
| Mobile Phase | Mobile phase: 18.3g sodium dihydrogen phosphate and 2.3g sodium dodecyl sulfate were added to 800mL water, mixed, ultrasonic degassed, pumped and filtered, and 8.3mL trethylamine was added to mix, pH was adjusted to 2.5 with phosphoric acid, wahaha water was added to 1000mL, and shaken well. Take 600mL of the above solution and 400mL chromatographic methanol mixture, ultrasonic degassing, obtained. 0.23% sodium dodecyl sulfate solution/methanol =60/40 |
| Detection | 220nm |
| Temperature | 25℃ |
| Flow Rate | 0.9ml/min |
| Injection Volume | 20ul |
| Note | / |
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