
Column | Ultisil® AQ-C18 (4.6×150mm,3μm) |
Mobile Phase | 5mmol/L ammonium acetate solution (containing 1% triethylamine, adjust pH=4.5 with glacial acetic acid)/acetonitrile=35/65 |
Detection | 262nm |
Temperature | 30℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 20 μL |
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