Determination of the Vitamin B6 in Food

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Vitamin B6, which includes pyridoxol, pyridoxal and pyridoxamine, is water-soluble in the form of phosphate in human body. As a component of some coenzymes in human body, it participates in many metabolic reactions, especially in amino acid metabolism. Clinical applicable vitamin B6 can prevent pregnancy vomiting and radiation vomiting. Yeast, liver, grain, meat, fish, eggs, beans and peanuts are particularly rich in vitamin B6.

Chromatographic Condition

ColumnUltisil® AQ-C18(4.6×150mm,5μm)
Mobile PhaseTime(min)Mobile phase A(%)Methanol(%)
 01000
 201000
 306535
 456238
 465050
 505050
 511000
 751000
WavelengthExcitation:293nmEmission: 395nm
Column Temp.30℃
Flow Rate1.0ml/min
Injection Volume10μL

Chromatogram and Data

SubstanceRetention timeAreaHeightPlatesTailing factorResolution
Pyridoxal15.295556411632708855125810.999
Pyridoxol22.436871490622970285133410.99610.801
Pyridoxamine36.6027323099792029694457811.08728.438
Total _21602122314882108 _ _ _

Conclusion

Using AQ-C18(4.6×150 mm,5μm) under this chromatographic condition can meet the test requirements.

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