Reference substance solution
| Column | Xtimate®C18(4.6×250mm，5μm) |
| Mobile Phase | 1% glacial acetic acid: add 10ml glacial acetic acid into 990ml Wahaha water to make 1% glacial acetic acid solution, shake well and degass with ultrasonic. 1% glacial acetic acid / methanol = 68 / 32 |
| Detection | 257nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 5ul |
| Note | / |
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