Joint pain relief cream is prepared from pepper flow extract, belladonna flow extract, peppermint oil, methyl salicylate, camphor, iodine, potassium iodide, diphenhydramine hydrochloride, etc. it has the effects of promoting blood circulation, diminishing inflammation and analgesia, and has the effect of dilating local blood vessels. Used for joint sprain and joint pain caused by cold and dampness.
According to the method in Volume 1 of Chinese Pharmacopoeia 2020, Welch Xtimate® C18 is tested, and the results can meet the testing requirements.

Chromatographic Condition

Column: Xtimate®C18(4.6×250mm,5μm)
Mobile phase: 1% ammonium sulfate solution/methanol=53/47;
Wavelength: 210nm;
Temperature: 30 ℃;
Flow rate: 1.0ml/min;
Injection volume: 10 μ L.


Diphenhydramine Hydrochloride Reference Solution

P/NRTAreaHeightPlate noAsymmetrySeparation
diphenhydramine hydrochloride13.54128.30346.8489811.23n.a

Test solution(Enlarge view)

P/NRTAreaHeightPlate noAsymmetrySeparation
diphenhydramine hydrochloride13.50141.38390.0295751.33n.a

Full scale diagram


Using Xtimate®C18(4.6×250mm,5μm) to measure under this chromatographic condition, it can meet the detection requirements.

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