Blank solution

ColumnBoltimate® LP-C18(
4.6×150 mm,2.7 μm)
Detection210nm
Temperature30℃
Flow Rate0.6ml/min
Injection Volume10ul
Note/

Mobile Phase

Mobile phase A; 281.1000mg of Butyl Ammonium Mobile phase A: weigh 1.42g citric acid, 2.31g disodium hydrogen phosphate, and dissolve and dilute with water, 1000ml (if necessary, adjust the pH value to 3.6 with phosphoric acid or sodium hydroxide solution), mix and filter,Readily available;

Time(min)Mobile phase A (%) Mobile phase B (%)
05446
74753
374753
902080
1102080
1115446
1205446

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