Blank solution
| Column | Xtimate® C18（4.6×150mm，3μm) |
| Detection | 300nm |
| Temperature | 40℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 5ul |
| Note | / |
Mobile Phase
Mobile phase A: weigh 1.74g of potassium dihydrogen phosphate, add water to dissolve and dilute to 1000ml, and use Adjust the pH value of phosphoric acid to 8.5 and filter it. Mobile phase B: tetrahydrofuran / acetonitrile = 10 / 90
| Time(min） | Mobile phase A (%) | Mobile phase B (%) |
| 0 | 95 | 5 |
| 3 | 92 | 8 |
| 10 | 90 | 10 |
| 14 | 85 | 15 |
| 16 | 78 | 22 |
| 30 | 61 | 39 |
| 35 | 61 | 39 |
| 40 | 95 | 5 |
| 50 | 95 | 5 |
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