System suitability solution – enlarged
| Column | Xtimate® C18（ 4.6×250mm，5μm) |
| Detection | 260nm |
| Temperature | 25℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
Mobile phase A: 0.05mol/L Ammonium acetate aqueous solution (pH7.0)
| Time(min） | Mobile phase A (%) | Methanol (%) |
| 0 | 98 | 2 |
| 10 | 95 | 5 |
| 22 | 78 | 22 |
| 32 | 72 | 28 |
| 40 | 35 | 65 |
| 50 | 35 | 65 |
| 51 | 98 | 2 |
| 65 | 98 | 2 |
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