ColumnUltisil® Alk-C18(4.6×250mm,5μm)
Mobile PhaseDissolve 1.32g of diammonium hydrogen phosphate in 750mL of water, adjust the
pH to 2.5 with phosphoric acid, add water to 1000mL, add 25mL of acetonitrile.
Detection210 nm
Flow Rate1.5 ml/min
Injection Volume50 μL

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