ColumnUltisil® Alk-C18(4.6×250mm,5μm)
Mobile PhaseDissolve 1.32g of diammonium hydrogen phosphate in 750mL of water, adjust the
pH to 2.5 with phosphoric acid, add water to 1000mL, add 25mL of acetonitrile.
Detection210 nm
Temperature25℃
Flow Rate1.5 ml/min
Injection Volume50 μL

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