| Column | Ultisil® Alk-C18（4.6×250mm，5μm) |
| Mobile Phase | Dissolve 1.32g of diammonium hydrogen phosphate in 750mL of water, adjust the pH to 2.5 with phosphoric acid, add water to 1000mL, add 25mL of acetonitrile. |
| Detection | 210 nm |
| Temperature | 25℃ |
| Flow Rate | 1.5 ml/min |
| Injection Volume | 50 μL |
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