Reference solution
| Column | Xtimate® C18（ 4.6×150mm，5μm) |
| Detection | 210nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
Mobile phase A; 281.1000mg of Butyl Ammonium hydrogen and a.1000ml of tetrahydrofluoric acid as mobile phase Water, mix well, filter, adjust the pH to 7.5 with 10% potassium hydroxide, and degass with ultrasonic
| Time(min） | Mobile phase A (%) | Acetonitrile (%) |
| 0 | 100 | 0 |
| 15 | 100 | 0 |
| 25 | 40 | 60 |
| 30 | 40 | 0 |
| 31 | 100 | 100 |
| 60 | 100 | 0 |
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