Reference solution

ColumnXtimate® C18(4.6×150mm,5μm)
Detection210nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume10ul
Note/

Mobile Phase

Mobile phase A: Take 1.69g tetrabutylammonium hydrogen sulfate and 2.28g dipotassium hydrogen phosphate, add 1000mL In water, mix and filter, adjust the pH to 7.5 with 10% potassium hydroxide, and degas by ultrasonic.

Time(min)Mobile phase A (%)Acetonitrile (%)
01000
151000
254060
30400
31100100
601000

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