Reference solution
| Column | Xtimate® C18（4.6×150mm，5μm) |
| Detection | 210nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
Mobile phase A: Take 1.69g tetrabutylammonium hydrogen sulfate and 2.28g dipotassium hydrogen phosphate, add 1000mL In water, mix and filter, adjust the pH to 7.5 with 10% potassium hydroxide, and degas by ultrasonic.
| Time(min） | Mobile phase A (%) | Acetonitrile (%) |
| 0 | 100 | 0 |
| 15 | 100 | 0 |
| 25 | 40 | 60 |
| 30 | 40 | 0 |
| 31 | 100 | 100 |
| 60 | 100 | 0 |
If you have any problem or require further information, please contact info@welchmat.com.
0 Comments