| Column | Ultisil® LP-C8（4.6×250mm，5μm) |
| Mobile Phase | See the table below for details |
| Detection | 285nm |
| Temperature | 40℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
Mobile Phase：
| Time (min) | 0.1% trifluoroacetic acid (%) | Acetonitrile (%) |
| 0 | 85 | 15 |
| 3 | 85 | 15 |
| 30 | 5 | 95 |
| 33 | 5 | 95 |
| 40 | 85 | 15 |
| 50 | 85 | 15 |
If you have any problem or require further information, please contact info@welchmat.com.
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