| Column | Ultisil® Alk C18（4.6×150mm，5μm) |
| Mobile Phase | 0.3% ammonium acetate/acetonitrile = 80/20 |
| Detection | 260nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 5 μL |
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