Sample solution 1(20ul)
| Column | Topsil® NH2（ 4.6×250mm，5μm) |
| Mobile Phase | Acetonitrile / water = 70 / 30 |
| Detection | Differential detector, temperature 40 ℃ |
| Temperature | 45℃ |
| Flow Rate | 1.5ml/min |
| Injection Volume | 20/10ul |
| Note | When the sample is prepared with water as solvent, there will be solvent effect when 20 μ L is injected. The solvent effect can be solved by reducing the injection amount (10 μ L) or changing the solvent. |
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