Sample solution 1(20ul)

ColumnTopsil® NH2(
4.6×250mm,5μm)
Mobile PhaseAcetonitrile / water = 70 / 30
DetectionDifferential detector, temperature 40 ℃
Temperature45℃
Flow Rate1.5ml/min
Injection Volume20/10ul
NoteWhen the sample is prepared with water as solvent, there will be
solvent effect when 20 μ L is injected. The solvent effect can be
solved by reducing the injection amount (10 μ L) or changing the
solvent.

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