| Column | Ultisil® ODS-3（4.6×150mm，3.5μm) |
| Mobile Phase | Mobile phase A: Take 3.9g of sodium dihydrogen phosphate dihydrate, add it to 1000mL of water, mix well, and adjust the pH to 2.5 with phosphoric acid; take 950mL of the above solution, mix it with 50mL of acetonitrile and shake well, filter it with suction, and degas it by ultrasonic. |
| Detection | 205nm |
| Temperature | 40℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
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