ColumnUltisil® ODS-3(4.6×150mm,3.5μm)
Mobile PhaseMobile phase A: Take 3.9g of sodium dihydrogen phosphate dihydrate, add it to 1000mL of water, mix well, and adjust the pH to 2.5 with phosphoric acid; take 950mL of the above solution, mix it with 50mL of acetonitrile and shake well, filter it with suction, and degas it by ultrasonic.
Flow Rate1.0 ml/min
Injection Volume20 μL


Leave a Reply

Avatar placeholder

Your email address will not be published. Required fields are marked *