Solution Diagram

Column | Ultisil® LP-C18( 4.6×300mm,5μm) |
Detection | 228nm |
Temperature | 40℃ |
Flow rate | 0.8ml/min |
Injection Volume | 10ul |
Note | / |
Mobile Phase
Time(Min) | 0.1% Phosphoric Acid (%) | Methanol (%) |
0 | 55 | 45 |
20 | 40 | 60 |
22 | 40 | 60 |
22.1 | 55 | 45 |
30 | 55 | 45 |
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