Solution Diagram
| Column | Ultisil® LP-C18（ 4.6×300mm，5μm） |
| Detection | 228nm |
| Temperature | 40℃ |
| Flow rate | 0.8ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
| Time(Min） | 0.1% Phosphoric Acid (%) | Methanol (%) |
| 0 | 55 | 45 |
| 20 | 40 | 60 |
| 22 | 40 | 60 |
| 22.1 | 55 | 45 |
| 30 | 55 | 45 |
If you have any problem or require further information, please contact info@welchmat.com.
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