Determination of Silymarin by Ultisil LP-C18

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Solution Diagram

ColumnUltisil® LP-C18(
4.6×300mm,5μm)
Detection228nm
Temperature40℃
Flow rate0.8ml/min
Injection Volume10ul
Note/

Mobile Phase

Time(Min)0.1% Phosphoric Acid (%)Methanol (%)
05545
204060
224060
22.15545
305545

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