Phytohormones are also called plant natural hormones or plant endogenous hormones. It refers to some organic compounds produced in plants that can regulate (promote, inhibit) their own physiological processes, which plays an important role in regulating the growth and development of plants.
Here Ultisil®Phenyl-Ether column was used to separate zeaxanthin, auxin, abscisic acid, ethylene, indoleic acid, agonist and naphthoacetic acid.
Conditions
| Column | Ultisil® Phenyl-Ether（4.6×250mm，5μm) |
| Wavelength | 260nm |
| Temp. | 30℃ |
| Flow rate | 1.0ml/min |
| Injection volume | 2μL |
| Mobile Phase | ||
|---|---|---|
| Time（min） | 0.1% Formic acid（%） | Acetonitrile（%） |
| 0 | 95 | 5 |
| 20 | 62 | 38 |
| 40 | 20 | 80 |
| 45 | 20 | 80 |
| 50 | 95 | 5 |
| 60 | 95 | 5 |
| Compund | Rt | Area | Height | Plates | Tailing factors | Resolution |
| ZT-Zeatin | 8.443 | 105664 | 18071 | 41157 | 1.150 | – |
| KT-Kinetin | 12.367 | 805820 | 112499 | 62322 | 1.311 | 21.524 |
| IAA-Auxin | 21.325 | 325163 | 41109 | 151639 | 1.028 | 42.945 |
| ABA-Abscisic acid | 21.974 | 1788837 | 234688 | 172656 | 1.023 | 3.014 |
| IBA-Indolic acid | 26.850 | 282336 | 37338 | 259993 | 1.027 | 23.101 |
| NAA-Naphthylacetic acid | 27.751 | 437711 | 56719 | 269447 | 1.024 | 4.246 |
| ACC-Ethylene | 33.382 | 67374 | 8335 | 364497 | 1.053 | 25.885 |
| _ | _ | 3812905 | 508759 |
If you have any problem or require further information, please contact info@welchmat.com.
0 Comments