Blank solvent
| Column | Xtimate® C18（ 4.6×250mm，5μm) |
| Mobile Phase | Phosphate buffer (ph8.5): weigh 6.0g anhydrous sodium dihydrogen phosphate and dissolve it in 50ml water. Prepare 1mol / L sodium dihydrogen phosphate solution; Weigh 7.1g anhydrous disodium hydrogen phosphate and dissolve it in 100ml water is made into 0.5mol/l disodium hydrogen phosphate solution; Take 1mol / L sodium dihydrogen phosphate solution 1.3ml, 0.5mol/l disodium hydrogen phosphate solution 32.5ml, add water to 1000ml, and use phosphoric acid or oxyhydrogen. Adjust the pH of sodium chloride solution to 8.5, mix well, filter by suction and degassing by ultrasonic; Phosphate buffer (ph8.5) / acetonitrile = 45 / 55 |
| Detection | 240nm |
| Temperature | 25℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
| Note | / |
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