Blank solvent

ColumnXtimate® C18(
4.6×250mm,5μm)
Mobile PhasePhosphate buffer (ph8.5): weigh 6.0g anhydrous sodium dihydrogen
phosphate and dissolve it in 50ml water. Prepare 1mol / L sodium
dihydrogen phosphate solution; Weigh 7.1g anhydrous disodium
hydrogen phosphate and dissolve it in 100ml water is made into
0.5mol/l disodium hydrogen phosphate solution; Take 1mol / L
sodium dihydrogen phosphate solution 1.3ml, 0.5mol/l disodium
hydrogen phosphate solution 32.5ml, add water to 1000ml, and use
phosphoric acid or oxyhydrogen. Adjust the pH of sodium chloride
solution to 8.5, mix well, filter by suction and degassing by
ultrasonic;

Phosphate buffer (ph8.5) / acetonitrile = 45 / 55
Detection240nm
Temperature25℃
Flow Rate1.0ml/min
Injection Volume20ul
Note/

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