Sample Solution (Full Scale)

ColumnXtimate® C18, 4.6×150mm, 5μm
Detection260 nm
Temperature35℃
Flow Rate0.8 mL/min
Injection Volume10 µL

Mobile Phase

Time(min)5 g/L ammonium acetate(%)Methanol/acetonitrile =9/1(%)
06040
143268
173268
221585
271585
286040
406040

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