Adaptive solution of relevant substance system

ColumnXtimate®C8 (5μm,4.6×250mm)
Mobile PhaseMethanol / acetonitrile / 0.075mol/l potassium dihydrogen phosphate
solution / 1.0mol/l citric acid solution = 30:30:36:4
And adjust the pH to 7.0 with 10mol / L sodium hydroxide solution
Detection254nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume10ul
Note/

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