| Column | Xtimate® C8 5μm 4.6×250mm |
| Mobile Phase | Methanol/acetonitrile /0.075mol/L potassium dihydrogen phosphate solution /1.0mol/L citrate solution =30:30:36:4 and 10mol/L sodium hydroxide solution was adjusted to PH 7.0 |
| Detection | 254nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 μL |
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