
Column | Ultisil® AQ-C18(4.6×250mm,5μm) |
Detection | 210nm |
Temperature | 35℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 20 μL |
Mobile Phase |
Time (min) | 40mm diammonium hydrogen phosphate/methanol=80/20 | acetonitrile/methanol=90/10 |
0 | 98 | 2 |
5 | 98 | 2 |
30 | 50 | 50 |
31 | 50 | 2 |
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