| Column | Ultisil® AQ-C18（4.6×250mm，5μm） |
| Detection | 210nm |
| Temperature | 35℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
Mobile Phase |
| Time (min) | 40mm diammonium hydrogen phosphate/methanol=80/20 | acetonitrile/methanol=90/10 |
| 0 | 98 | 2 |
| 5 | 98 | 2 |
| 30 | 50 | 50 |
| 31 | 50 | 2 |
If you have any problem or require further information, please contact info@welchmat.com.
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