| Column | Welch Ultisil ® C18（ 4.6×250mm，5μm) |
| Mobile Phase | Mobile Phase A: Take 1 mL of triethylamine and 1 mL of trifluoroacetic acid, add them to 1000 mL of purified water, mix well, and degas using ultrasound to obtain the solution. Time(min) Mobile Phase A(%) Acetonitrile (%) 0 75 25 20 45 55 25 15 85 30 15 85 31 75 25 45 75 25 |
| Column Temperature | 35 ℃ |
| Detection wavelength | 225 nm |
| Flow Rate | 1.1 ml/min |
| Injection Volume | 10 μL |
| Note | / |
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