ColumnUltisil® Phenyl-Ether(4.6×250mm,5μm)
Mobile PhaseSee the table below for details
Detection196nm
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume20 μL

Mobile Phase:

Mobile phase A: Weigh 3.55g of disodium hydrogen phosphate, dissolve it in 1000ml of Wahaha water, adjust the pH to 5.0 with phosphoric acid, mix well, filter with suction, and degas by ultrasonic Mobile phase B: Acetonitrile

Time (min)Mobile phase A(%)Mobile phase B(%)
01000
221000
358515
557030
607030
611000
801000

If you have any problem or require further information, please contact info@welchmat.com.


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