| Column | Ultisil® Polar-RP（4.6×150mm，3μm) |
| Mobile Phase | See the table below for details |
| Detection | 220nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
Mobile Phase:
| Time (min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 85 | 15 |
| 45 | 10 | 90 |
| 50 | 10 | 90 |
| 51 | 85 | 15 |
| 55 | 85 | 15 |
If you have any problem or require further information, please contact info@welchmat.com.
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