Determination of related substances in pilocarpine nitrate eye drops by Ultis-Polar-RP

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ColumnUltisil® Polar-RP（4.6×250mm，5μm)
Mobile PhaseMethanol/acetonitrile/0.002mol/L tetrabutylammonium hydroxide
solution=55/60/885 (pH7.7)
Detection220nm
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume20 μL

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