
Column | Ultisil® Polar-RP(4.6×250mm,5μm) |
Mobile Phase | Methanol/acetonitrile/0.002mol/L tetrabutylammonium hydroxide solution=55/60/885 (pH7.7) |
Detection | 220nm |
Temperature | 30℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 20 μL |
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