Reference standard

Public draft of the revised draft of the national drug standard for vitamin E milk.

Chromatographic conditions

Column: Xtimate® C18 (4.6×250mm, 5μm)

Mobile phase: methanol/water=98/2

Detection wavelength: 284nm

Column temperature: 35℃

Flow rate: 1.0mL/min

Injection volume: 20μL

Chromatograms and data

  1. Related substances

1.1 System suitability solution

NumRetention timePeak areaPeak heightNumber of platesAsymmetryDegree of separation
115.5350.8782.631149720.824.44
218.1600.6261.495115090.923.82
320.81570.780150.199134660.85n.a.

1.2 A-tocopherol reference solution

NumRetention timePeak areaPeak heightNumber of platesAsymmetryDegree of separation
115.5100.9532.686129960.82n.a.

1.3 Sensitivity solution

NumRetention timePeak areaPeak heightNumber of platesAsymmetryDegree of separation
120.8650.6911.444129040.84n.a.

1.4 Test solution (related substances)

1.4.1 Enlarged view

NumRetention timePeak areaPeak heightNumber of platesAsymmetryDegree of separation
14.8750.0470.400148301.103.70
25.4450.0130.142213740.9814.21
310.0920.0240.07863400.793.01
411.4600.0240.095127741.044.06
513.2680.0270.077118390.735.04
615.5450.0180.060221431.041.56
716.2520.0250.076174390.982.53
817.4630.0340.122222100.801.28
918.1950.6071.460116470.962.16
1019.2400.0090.038680551.193.24
1120.87269.023146.985136430.852.24
1222.2320.0230.067314961.024.98
1325.0630.0220.042245560.953.66
1427.0900.0110.028527251.10n.a.

1.4.2 Full scale diagram

2. Content determination

NumRetention timePeak areaPeak heightNumber of platesAsymmetryDegree of separation
120.85214.29629.879129910.84n.a.

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