Determination of related substances and content of vitamin B2 tablets by Xtimate C18

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ColumnXtimate® C18（4.6×250mm，5μm)
Mobile Phase0.01mol/L sodium heptanesulfonate in 0.5% glacial acetic
acid/acetonitrile/methanol=85/10/5
Detection444nm
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume20 μL

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