Reference substance of impurityC
Column | Welch Ultisil® HILIC Amide(4.6*250mm,5um) |
Mobile Phase | Phosphoric acid buffer (dissolve 6.8g potassium dihydrogen in water and diluted to 1000 mL) – acetonitrile = 25:75 |
Wavelength | 210nm |
Temperature | 45℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 20 μl |
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