Determination of Related Substance of Ascorbic Acid Injection

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Reference substance of impurityC

ColumnWelch Ultisil® HILIC Amide(4.6*250mm,5um)
Mobile PhasePhosphoric acid buffer (dissolve 6.8g potassium dihydrogen in water and diluted to 1000 mL) – acetonitrile = 25:75
Wavelength210nm
Temperature45℃
Flow Rate1.0 ml/min
Injection Volume20 μl

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