Mixed reference solution
| Column | Ultisil® XB-C18（ 4.6×250mm，5μm) |
| Detection | 210nm |
| Temperature | 35℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
| Time(min） | Potassium dihydrogen phosphate buffer (%) | Acetonitrile (%) |
| 0 | 80 | 20 |
| 5 | 80 | 20 |
| 15 | 65 | 35 |
| 50 | 25 | 75 |
| 55 | 25 | 75 |
| 56 | 80 | 20 |
| 70 | 80 | 20 |
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