
Column | Xtimate® C18(4.6×250mm,5μm) |
Mobile Phase | Phosphate buffer: take dipotassium hydrogen phosphate, prepare 0.01oml/L with water, add 10ml of 25% ammonium hydroxide tetrabutyrate solution to each 1000ml, mix well, adjust the pH value to 7.0 with phosphoric acid, mix well, and filter with suction , that is. Phosphate buffer/acetonitrile = 70/30 |
Detection | 205nm |
Temperature | 30℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 20 μL |
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