| Column | Xtimate® C18（4.6×250mm，5μm) |
| Mobile Phase | Phosphate buffer: take dipotassium hydrogen phosphate, prepare 0.01oml/L with water, add 10ml of 25% ammonium hydroxide tetrabutyrate solution to each 1000ml, mix well, adjust the pH value to 7.0 with phosphoric acid, mix well, and filter with suction , that is. Phosphate buffer/acetonitrile = 70/30 |
| Detection | 205nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
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