Chromatogram and Data

ColumnXtimate®C18(
4.6×250mm,5μm)
Mobile PhasePhosphate buffer: take dipotassium hydrogen phosphate, prepare
0.01oml/L with water, add 10ml of 25% ammonium hydroxide
tetrabutyrate solution to each 1000ml, mix well, adjust the pH value
to 7.0 with phosphoric acid, mix well, and filter with suction , that is.
Phosphate buffer/acetonitrile = 70/30
Detection250nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume20ul
Note/

If you have any problem or require further information, please contact info@welchmat


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