ColumnXtimate® C18(4.6×250mm,5μm)
Mobile PhasePhosphate buffer: take dipotassium hydrogen phosphate, prepare 0.01oml/L with
water, add 10ml of 25% ammonium hydroxide tetrabutyrate solution to each
1000ml, mix well, adjust the pH value to 7.0 with phosphoric acid, mix well, and
filter with suction , that is.
Phosphate buffer/acetonitrile = 70/30
Flow Rate1.0 ml/min
Injection Volume20 μL

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